![]() Barcoding: When sticking a barcode on the glass slide, ensure that a marginal space of 1-2 mm remains on all sides between the sticker and the label area outer limits.In case of using a 40x objective, adjust the correlation ring to the thickness of the coverslip. For best results, the distance between slide edges and the edges of the mounted coverslip should be 1-2 mm, and the edges should be parallel with each other. Also, make sure that there are no air bubbles under the coverslip, as this also may result in areas scanned out of focus. However, make sure that the plastic coverslip is not twisted or wrinkled as this may affect scanner focus. Coverslipping: Glass and plastic coverslips may both be used.Also, use slides with standard sizes (75-76 mm length and 25-26 mm width for standard slides or 51-52 mm width for double-width slides). Avoid any folds or wrinkles in the tissue for optimal focus. ![]() Fixing on glass slides: Digital slide scanners produce best results with flat tissue surfaces.Staining: Avoid pale staining or strong background staining because they are likely to hinder automated tissue detection.If you have thicker samples, extended focus scanning or Z-stack scanning will produce best results. Sectioning: For single-layer scanning, we recommend a standard tissue thickness of 7-10 μm.Please find below some important points that need to be considered when preparing tissue specimens for scanning. CentralLogService Application (CLS) – CybersecurityĮven the world’s best digital slide scanner cannot compensate for a poor-quality tissue sample, so a great quality digital slide starts with well-prepared tissue. ![]()
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